Monday, March 9, 2009

How to extract D.N.A. from a strawberry

D.N.A. is a molecule that contains a genetic code in the cells of plants and animals. Extracting D.N.A from a strwberry is a multi-step process. In order to extract the D.N.A. you need a heavy duty ziplock bag a fresh strawberry, a cheesecloth, a funnel, a testube, a wooden coffee stirer, a hundred mL beaker, some cold rubbing alchohol, (100mL of shampoo(without conditioner) and 900 mL of water)this is your extraction buffer. You start the procedure by placing the strawberry in a ziploc bag which you then smash for 2 minutes. Then you add 10mL of your extraction buffer to the ziploc bag then smash it again for one minute. Filter your solution in your ziploc bag through the cheesecloth then pour it into a testube so it is 1/8 full. Finally slowly pour the cold rubbing alchohol into the testube. You should see the D.N.A. foam out at the top. An explanation for this is that the soap breaks down the cell membranes in a cell. Then the alchohol percipitates th D.N.A making it visible.

Information found here.

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